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KMID : 0366119770050030141
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Korean Journal of Applied Microbiology & Bioengineering
1977 Volume.5 No. 3 p.141 ~ p.151
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The Physiological and Biochemical Studies of Nocardia sp
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Abstract
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Distribution of tellurite- and tellurate-reducing enzymes in the cell of Nocardia sp, the purification and the chemical properties of enzymes were investigated. Tellurite- and tellurate-reducing enzymes were located in the cytoplasm, but T. T. C. reduction part was in the cell membrane.
Purification of tellurite- and tellurate-reducing enzymes was possible with the application of ammonium sulfate precipitation method and DEAE-Cellulose or CM-Cellulose column chromatographic method from the crude soluble part of the cell.
On investigating the properties of purified enzyme, one of NADP, NADPH and reductive methylene blue (leucomethylene blue) was thought to react as a hydrogen donor. Both NADH and NADPH, or either of them would be physiological hydrogen donor.
In the reaction of this enzyme, either tellurite or tellurate reacts as a hydrogen acceptor, but on the other hand either selenite or selenate also reacts as a hydrogen acceptor.
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